PEATmoss

RNA-seq Developmental stages gmv3.3

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Gene models: v3.3

Description: Expression data from several developmental stages for Gransden WT and Reute WT ("Dry spores" and "leaflets - Ksl" have 2 replicates and the "imbibed spores - BlqA" only have one. All other experiments have 3 replicates). Dry spores and imbibed spores from Grandsen and protonema Klq, gametophore ksl and adult gametophore ksl from Reute are unpublished data. The rest of the experiments were published in Perroud et al., 2018

Abbreviations
BCD liquid	Blq
BCDA (ammonium) liquid	BlqA
BCD solid	Bsl
BCDA (ammonium) solid	BslA
Gibberellin A9 methyl-ester	GA9
hydroponic	hydr
Knop liquid	Klq
Knop liquid ammonium	KlqA
Knop solid	Ksl
12-oxophytodienoic acid	OPDA

Note: OPDA is a precursor of jasmonic acid.

Figure 1. Sporophyte developmental stages

Abb.	Developmental stage	Description
E1	Embryo 1:	Developing embryo; the upper, chloroplast rich half will develop into the spherical, spore-containing spore capsule, whereas the lower part will connect to the gametophore for nourishment of the developing sporophyte.
E2	Embryo 2:	Elongated embryo without developed stomata. Connection between gametophore and sporophyte still loose but cells started to differentiate.
ES	Early sporophyte:	Stomata are developed, clear separation of capsule (inflation) and developing seta (2n).
PM	Premeiotic sporophyte:	Spherical green translucend sporophyte containing spore mother cells (2n), seta starts to turn brownish.
M	Meiotic sporophyte:	Opaque green sporophyte containing tetrades which will later form each four spores (1n, meiosis occured).
Y	Yellow sporophyte:	Contains early spores of the final size covered by a plasma membrane, seta darkend during the maturation process.
LB	Light brown sporophyte:	Spores started to mature, the spore wall thickens by exine deposition.
B	Brown sporophyte:	Spores are mature, spore wall consists of a thick perine layer with spikes, exine, seperating layer and intine, sporophyte detaches easily.

The next figures represent the available samples in this data set. The color in the colored part of the figures below will be replaced by the expression color (from the white-yellow-orange-red color scale) when using the Expression Viewer.